Strain Data Sheet

RBRC10321

Strain Information

Image
BRC No.RBRC10321
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129.129P2-Aldh2<tm1Kaw>
Former Common nameAldh2 KO mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Kyoko Kitagawa, University of Occupational and Environmental Health in 1998. E14 ES cells were used. The mutant mice were backcrossed to 129 over 7 times.
Strain descriptionAldh2 gene knockout mice. A termination codon and a neomycin selection cassette was inserted into exon 3 of Aldh2 gene.
Colony maintenance
References
Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse.
K Kitagawa, T Kawamoto, N Kunugita, T Tsukiyama, K Okamoto, A Yoshida, K Nakayama, K Nakayama
FEBS Lett., 476, 306-311 (2000). 10913633

Aldehyde dehydrogenase 2 gene targeting mouse lacking enzyme activity shows high acetaldehyde level in blood, brain, and liver after ethanol gavages.
Toyohi Isse, Koji Matsuno, Tsunehiro Oyama, Kyoko Kitagawa, Toshihiro Kawamoto
Alcohol Clin. Exp. Res. 29(11), 1959-1964 (2005). 16340452

An essential role for mitochondrial aldehyde dehydrogenase in nitroglycerin bioactivation.
Zhiqiang Chen, Matthew W Foster, Jian Zhang, Lan Mao, Howard A Rockman, Toshihiro Kawamoto, Kyoko Kitagawa, Keiichi I Nakayama, Douglas T Hess, Jonathan S Stamler
Proc. Natl. Acad. Sci. USA, 102, 12159-12164 (2005). 16103363

Characteristics of aldehyde dehydrogenase 2 (Aldh2) knockout mice.
Hsu-Sheng Yu, Tsunehiro Oyama, Toyohi Isse, Kyoko Kitakawa, Masanori Ogawa, Thi-Thu-Phuong Pham, Toshihiro Kawamoto
Toxicol. Mech. Methods., 19(9), 535-540 (2009). 19874182

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aldh2
MGI:99600		aldehyde dehydrogenase 2, mitochondrial5Aldh2<tm1Kaw>
MGI:2157245		targeted mutation 1, Toshihiro Kawamoto
  • alcohol sensitivity, acute(MedGEN)
    • neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA5

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cellular respiration (MP:0010954)

  • abnormal enzyme/coenzyme activity (MP:0005584)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Aldh2 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data