Strain Data Sheet

RBRC10320

Information1

Image
BRC No.RBRC10320
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Kdm2a<tm1.1Hko>
Former Common nameFbxl11(Kdm2A)cond.
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN in 2009. M1 ((129 x C57BL/6)F1) ES cells were used. This strain was backcrossed to C57BL/6J twice. A neo cassette was removed by crossing a Flp mouse strain.
Strain descriptionKdm2a (lysine (K)-specific demethylase 2A, Fbxl11) flox mouse. A pair of loxP flank exon 8 of the Kdm2a.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kdm2alysine (K)-specific demethylase 2A19Kdm2a<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Information2

Donor DNAyeast FRT (flipase recombination target) site, phage P1 loxP sites, mouse Kdm2a genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data