Strain Data Sheet

RBRC10317

Information1

Image
BRC No.RBRC10317
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Epc1<tm1Hko>
Former Common nameEpc1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Haruhiko Koseki and Kyo-ichi Isono, RIKEN, in 2014. R1((129X1/SvJ x 129S1/Sv)F1) ES cells were used. This strain was backcrossed to C57BL/6J twice.
Strain descriptionEpc1 (enhancer of polycomb homolog 1) knockout mice.
Colony maintenance
ReferencesMol. Cell Biol., 37(19) (2017). 28694333

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Epc1enhancer of polycomb homolog 118Epc1<tm1Hko>targeted mutation 1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, mouse PGK promoter, E.coli neo, PGK pA, mouse Epc1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data