Strain Data Sheet

RBRC10308

Information1

Image
BRC No.RBRC10308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nfil3<tm1.2Kubo>
Former Common nameE4bp4 floxed mouse, Nfil3 floxed mouse
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masato Kubo, RIKEN Yokohama Institute in 2010. M1/M-1 ES cells derived from (129 x C57BL/6)F1 were used. Mice were backcrossed with C57BL/6JJcl for 8 generations. Neo gene fragment was deleted by crossing with a FLPe mouse line in B6 background before backcrossing.
Strain descriptionFloxed mouse for Nfil3 (nuclear factor, interleukin 3, regulated; alternative name is E4bp4).
Colony maintenance
ReferencesNat Immunol, 12 (5), 450-459 (2011). 21460847

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nfil3nuclear factor, interleukin 3, regulated13Nfil3<tm1.2Kubo>targeted mutation 1.2, Masato Kubo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site13Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Information2

Donor DNAEnterobacteria phage P1 loxP sites, Saccharomvces cerevisiae FRT site, mouse Nfil3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Immunol, 12 (5), 450-459 (2011).
DepositorMasato Kubo (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data