Strain Data Sheet

RBRC10305

Information1

Image
BRC No.RBRC10305
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6;B6D2-Lrit2<em1Tfur>
Former Common nameLrit2 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2015. B6D2HF1-derived fertilized eggs were used and mice were crossed with C57BL/6J. Mixed genetic background.
Strain descriptionLrit2(leucine-rich repeat, immunoglobulin-like and transmembrane domains 2)mutant mice generated by the CRISPR/Cas9. One bp insertion at exon 2, resulting in conversion of Asn51 to stop codon.
Colony maintenance
ReferencesCell Reports, 22, 3548-3561 (2018). 29590622

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrit2leucine-rich repeat, immunoglobulin-like and transmembrane domains 214Lrit2<em1Tfur>endonuclease-mediated mutation 1, Takahisa Furukawa

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 22, 3548-3561 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data