Strain Data Sheet

RBRC10304

Information1

Image
BRC No.RBRC10304
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Lrit1/Lrit2<tm1Tfur>
Former Common nameLrit1/2-DKO
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2017. JM8.N4 ES cells derived from C57BL/6N were used and mice were crossed with C57BL/6J. C57BL/6 genetic background. Mutation of Rd8(Crb1) was replaced with its normal allele.
Strain descriptionKO mice for genes, Lrit1(leucine-rich repeat, immunoglobulin-like and transmembrane domains 1)and Lrit2(leucine-rich repeat, immunoglobulin-like and transmembrane domains 2). Genomic region from Exon 2 of Lrit1 to Exon 2 of Lrit2 was replaced with a neo gene. Homozygous mutants exhibit abnormality of synapse formation between cone photoreceptor cells and bipolar cells of retina.
Colony maintenance
ReferencesCell Reports, 22, 3548-3561 (2018). 29590622

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrit1leucine-rich repeat, immunoglobulin-like and transmembrane domains 114Lrit1<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrit2leucine-rich repeat, immunoglobulin-like and transmembrane domains 214Lrit2<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAyeast FRT (flipase recombination target) sites, Mouse phosphoglycerate kinase promoter, E.coli Neomycin resistance gene, SV40 PolyA signal, Bacteriophage P1 loxP site, Mouse Lrit1 genomic DNA, Mouse Lrit2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 22, 3548-3561 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data