Strain Data Sheet


Strain Information

BRC No.RBRC10303
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC3;B6-Lrit1<tm1b(EUCOMM)Hmgu>/Tfur
Former Common nameLrit1-gt
H-2 Haplotype
ES Cell lineJM8.N4 [C57BL/6N]
Background strain
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2017. JM8.N4 ES cells derived from C57BL6/N were used. C3H and B6 mixed genetic background. Mutations of Rd1(Pde6b) and Rd8(Crb1) were replaced with their normal alleles.
Strain descriptionLrit1(leucine-rich repeat, immunoglobulin-like and transmembrane domains 1)mutant mice.A pair of loxP sites flank Exon 2. FRT IRES-LacZ loxP Neo FRT loxP DNA fragment was inserted at intron 1 of the Lrit1 and another loxP was placed at intron 2. Homozygous mutants exhibit abnormality of synapse formation between cone photoreceptor cells and bipolar cells of retina. loxP Neo FRT loxP and Exon 2 DNA fragment was removed by Cre.
Colony maintenance
ReferencesCell Reports, 22, 3548-3561 (2018). 29590622

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrit1leucine-rich repeat, immunoglobulin-like and transmembrane domains 114Lrit1<tm1b(EUCOMM)Hmgu>targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Ordering Information

Donor DNAYeast FRT site, Mouse En2 intron, encephalomyocarditis virus IRES, E.coli lacZ, SV40 PolyA signal, Bacteriophage P1 loxP site, mouse Lrit1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 22, 3548-3561 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University. The RECIPIENT shall acknowledge the EUCOMM as the Provider of the ES cells to generate KO mice.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data