Strain Data Sheet

RBRC10293

Strain Information

Image
BRC No.RBRC10293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Itpr2<tm2.1Kmik>
Former Common nameItpr2 floxed mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2018. ES cells derived from C57BL/6 were used.
Strain descriptionItpr2 (inositol 1,4,5-trisphosphate receptor 2) floxed mice. A neo gene fragment was removed by crossing with a Flp mouse line.
Colony maintenance
ReferencesJ. Clin. Invest., 124(11) 4773-4780 (2014). 25329695

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Itpr2inositol 1,4,5-triphosphate receptor 26Itpr2<tm2.1Kmik>targeted mutation 2.1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Itpr2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Zhang M, Zhi N, Feng J, Liu Y, Zhang M, Liu D, Yuan J, Dong Y, Jiang S, Ge J, Wu S, Zhao X.
ITPR2 Mediated Calcium Homeostasis in Oligodendrocytes is Essential for Myelination and Involved in Depressive-Like Behavior in Adolescent Mice.
Adv Sci (Weinh) e2306498(2024) 38476116