Strain Data Sheet

RBRC10289

Strain Information

Image
BRC No.RBRC10289
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Itpr2<tm1Kmik>
Former Common nameIP3R2 knockout mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. J1 ES cells derived from 129S4/SvJae were used. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations.
Strain descriptionItpr2 (inositol 1,4,5-trisphosphate receptor 2) KO mouse. A nls-LacZ gene fragment was inserted downstream of the translation initiation codon. Homozygous mutant exhibit decreased sweating and disturbed calcium signaling in sweat glands.
Colony maintenance
References
IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism.
Futatsugi A, Nakamura T, Yamada M K, Ebisui E, Nakamura K, Uchida K, Kitaguchi T, Takahashi-Iwanaga H, Noda T, Aruga J, Mikoshiba K
Science, 309(5744) 2232-2234 (2005). 16195467

Abolished InsP3R2 function inhibits sweat secretion in both humans and mice.
Klar J, Hisatsune C, Baig S M, Tariq M, Johansson A C, Rasool M, Malik N A, Ameur A, Sugiura K, Feuk L, Mikoshiba K, Dahl N
J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695

Adrenergic inhibition facilitates normalization of extracellular potassium after cortical spreading depolarization.
Monai H, Koketsu S, Shinohara Y, Ueki T, Kusk P, Hauglund N L, Samson A J, Nedergaard M, Hirase H
Sci. Rep., 11, 8150 (2021). 33854148

Health Report

Examination Date / Room / Rack2025/09/08Room:3-CRack:FSentinel mouse program
2025/06/30Room:3-CRack:FSentinel mouse program
2025/04/21Room:3-CRack:FSentinel mouse program
2025/03/05Room:3-CRack:FAdditional tests
2025/01/20Room:3-CRack:FSentinel mouse program
2024/10/21Room:3-CRack:FSentinel mouse program
2024/04/22Room:3-CRack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Itpr2
MGI:99418		inositol 1,4,5-triphosphate receptor 26Itpr2<tm1Kmik>
MGI:3604623		targeted mutation 1, Katsuhiko Mikoshiba
  • isolated anhidrosis with normal sweat glands(MedGEN)
    • lacZ beta-galactosidase (E. coli)6
    neo neomycin resistance gene6 mouse phosphoglycerate kinase promoter (PGK promoter)
    nls SV40 large T antigen nuclear localization signal (NLS)6nls

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal sweat gland physiology (MP:0013368)

  • hypohidrosis (MP:0013371)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse PGK promoter, Escherichia coli neomycin-resistant gene, mouse PGK pA, SV40 large tumor nuclear location signal, Escherichia coli lacZ gene, mouse Itpr2 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Vittani M, Herlo R, Wang X, Christensen MDB, Vo CT, Mishima T, Kusk P, Konno A, Hirai H, Tsuboi T, Kitaguchi T, Kjaerby C, Asiminas A, Yokoyama T, Sakamoto M, Nedergaard M, Hirase H.
    Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP3R2-mediated Ca2+ signaling in mice.
    Proc Natl Acad Sci U S A 122(27) e2422069122(2025) 40591593