RBRC10269

Information1

Image
BRC No.RBRC10269
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-floxed EGFP,-rtTA,-tTS)1Kyot
Former Common nameB6-cTg/rtTA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2020/03/30Room:4-CRack:B
2020/03/09Room:4-CRack:B
2020/03/02Room:4-CRack:B
2020/01/27Room:4-CRack:B
2019/11/25Room:4-CRack:B
2019/09/30Room:4-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)4EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)4GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)4nls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
rtTAE. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)4rtTA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTSE. coli Tetracycline repressor (tetR), rat Kid1 (ZFP KRAB domain) cDNA4tTS

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA4

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 loxP sites, SV40 large T antigen nuclear localization signal (NLS), Jellyfish EGFP cDNA, SV40 polyA signal, E. coli TetR mutant (rtTA), Synthetic DNA (Synthetic intron sequence), EMC (encephalomyocarditis virus)internal ribosome entry site, tTS (E. coli Tetracycline repressor (tetR), rat Kid1 (ZFP KRAB domain) cDNA), bovine growth hormone polyA signal
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Itoh (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data