Strain Data Sheet

RBRC10268

Information1

Image
BRC No.RBRC10268
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mlc1<tm1.1kftnk>
Former Common nameMlc1 tetO knockin
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Kazuhiro Ikenaka, National Institute of Physiological Sciences, and Kenji Tanaka, Keio University. ES cells derived from 129S6/B6 hybrid were used. Mixed genetic background.
Strain descriptionA mutant mouse in which the tetracycline operator (tetO) was inserted upstream of the translation initiation site. This strain was generated by crossing RBRC10267 with a Flpe line.
Colony maintenance
ReferencesGlia, 65(1), 150-168 (2017). doi: 10.1002/glia.23084. Epub 2016 Oct 17. 27748972

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mlc1megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)11Mlc1<tm1.1kftnk>targeted mutation 1.1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)11tetOCMV promoter

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, E. coli Tetracycline operator site, CMV minimal promoter, mouse Mlc1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorKazuhiro Ikenaka (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data