Strain Data Sheet

RBRC10268

Strain Information

Image
BRC No.RBRC10268
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mlc1<tm1.1kftnk>
Former Common nameMlc1 tetO knockin
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Kazuhiro Ikenaka, National Institute of Physiological Sciences, and Kenji Tanaka, Keio University. ES cells derived from 129S6/B6 hybrid were used. Mixed genetic background.
Strain descriptionA mutant mouse in which the tetracycline operator (tetO) was inserted upstream of the translation initiation site. This strain was generated by crossing RBRC10267 with a Flpe line.
Colony maintenance
References
Astrocyte-mediated infantile-onset leukoencephalopathy mouse model.
Sugio S, Tohyama K, Oku S, Fujiyoshi K, Yoshimura T, Hikishima K, Yano R, Fukuda T, Nakamura M, Okano H, Watanabe M, Fukata M, Ikenaka K, Tanaka K F
Glia, 65(1), 150-168 (2017). doi: 10.1002/glia.23084. Epub 2016 Oct 17. 27748972

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
Mlc1
MGI:2157910
megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)11Mlc1<tm1.1kftnk> targeted mutation 1.1, Kenji Tanaka
  • megalencephalic leukoencephalopathy with subcortical cysts 1(MedGEN)

  • megalencephalic leukoencephalopathy with subcortical cysts(DisGeNET)
  • loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP
    tetO tetracycline operator (E. coli)11tetO CMV promoter

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal astrocyte morphology(MP:0002182)

  • abnormal brain white matter morphology(MP:0008026)

  • abnormal cerebellum morphology(MP:0000849)

  • abnormal myelin sheath morphology(MP:0003871)

  • behavior/neurological phenotype(MP:0005386)
  • more 5 phenotypes
  • brain vacuoles(MP:0008025)

  • decreased myelin sheath amount(MP:0020456)

  • demyelination(MP:0000921)

  • dysmyelination(MP:0013438)

  • edema(MP:0001785)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, E. coli Tetracycline operator site, CMV minimal promoter, mouse Mlc1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Tet system
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorKazuhiro Ikenaka (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data