Strain Information | |
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Image | |
BRC No. | RBRC10268 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Mlc1<tm1.1kftnk> |
Former Common name | Mlc1 tetO knockin |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed and deposited by Kazuhiro Ikenaka, National Institute of Physiological Sciences, and Kenji Tanaka, Keio University. ES cells derived from 129S6/B6 hybrid were used. Mixed genetic background. |
Strain description | A mutant mouse in which the tetracycline operator (tetO) was inserted upstream of the translation initiation site. This strain was generated by crossing RBRC10267 with a Flpe line. |
Colony maintenance | |
References | Astrocyte-mediated infantile-onset leukoencephalopathy mouse model. Sugio S, Tohyama K, Oku S, Fujiyoshi K, Yoshimura T, Hikishima K, Yano R, Fukuda T, Nakamura M, Okano H, Watanabe M, Fukata M, Ikenaka K, Tanaka K F Glia, 65(1), 150-168 (2017). doi: 10.1002/glia.23084. Epub 2016 Oct 17. 27748972 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 11 | Frt | ||||
Mlc1 MGI:2157910 | megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human) | 11 | Mlc1<tm1.1kftnk> | targeted mutation 1.1, Kenji Tanaka | |||
loxP | phage P1 loxP | 11 | loxP | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
tetO | tetracycline operator (E. coli) | 11 | tetO | CMV promoter |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 5 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, E. coli Tetracycline operator site, CMV minimal promoter, mouse Mlc1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Tet system |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Kazuhiro Ikenaka (National Institute for Physiological Sciences) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |