Strain Data Sheet

RBRC10267

Information1

Image
BRC No.RBRC10267
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mlc1<tm1kftnk>
Former Common nameMlc1 STOPtetO knockin
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Kazuhiro Ikenaka, National Institute of Physiological Sciences, and Kenji Tanaka, Keio University. ES cells derived from 129S6/B6 hybrid were used. Mixed genetic background.
Strain descriptionA mutant mouse in which a construct containing a loxP site, FRT site, neo cassette, STOP, FRT site, tetracycline operator (tetO) and loxP site was inserted upstream of the translation initiation site. Homozygous mutants show vacuolation in the white matter at 12 months of age.
Colony maintenance
ReferencesGlia, 65(1), 150-168 (2016). doi: 10.1002/glia.23084. Epub 2016 Oct 17. 27748972

Health Report

Examination Date / Room / Rack2022/07/11Room:3-BRack:J
2022/04/11Room:3-BRack:J
2022/01/24Room:3-BRack:J
2021/10/26Room:3-BRack:J
2021/07/26Room:3-BRack:J
2021/04/26Room:3-BRack:J
2021/02/01Room:3-BRack:J
2020/11/09Room:3-BRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mlc1megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)11Mlc1<tm1kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
His3His3 (S. cerevisiae)11His3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)11tetOCMV promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA11

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) sites, mouse PGK promoter, E. coli EM7 promoter, E. coli Neomycin resistance gene, yeast His3 3' UTR, SV40 polyA signal, E. coli Tetracycline operator site, CMV minimal promoter, mouse Mlc1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorKazuhiro Ikenaka (National Institute for Physiological Sciences)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data