Strain Data Sheet

RBRC10266

Information1

Image
BRC No.RBRC10266
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ppm1l<tm1Tkoba>/79
Former Common namePpm1llox/wt mouse, PP2Cεlox/wt mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Motoko Ohnishi, Chubu University. Developed by Shinri Tamura, Tohoku University, in collaboration with RIKEN CDB in 2006-2007 (CDB0428K). This strain was backcrossed to C57BL/6N over 28 generations.
Strain descriptionPpm1l [protein phosphatase 1 (formerly 2C)-like] knockout mice. The b-galactosidase(LacZ) and neomycin gene cassette were inserted into the 50 UTR sequence of exon 1. Homozygous mutants were born less than the mendelian ratio and died by 4 weeks old. ES clone #79 was used to generate this line.
Colony maintenance
ReferencesFEBS Lett., 590(20), 3606-3615 (2016). 27680501

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ppm1lprotein phosphatase 1 (formerly 2C)-like3Ppm1l<tm1Tkoba>targeted mutation 1, Takayasu Kobayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence3lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 lox71 site, E. coli lacZ, rabbit beta-globin 3’end, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neo, phage P1 loxP site, PGK3’end, mouse Ppm1l genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., Oct, 590(20), 3606-3615 (2016).
DepositorMotoko Ohnishi (Chubu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data