Strain Data Sheet

RBRC10262

Information1

Image
BRC No.RBRC10262
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6J.Cα-IRES Venus KI, Vα14, Vb8.1 clone
Former Common nameNKT clone
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2010. ES cells were established by the nuclear transfer of NKT cells into enucleated eggs derived from B6D2F1. IRES-Venus was inserted downstream of the TCR Ca stop codon in the NKT-ES cells. Chimeric mice were generated and then backcrossed with C57BL/6J for 10 generations.
Strain descriptionCloned mice harboring invariant TCR chains, Va14-Ja18 and Vb8.1. Venus expression from the TCRa locus.
Colony maintenance
ReferencesBlood, 115(2), 230-237 (2010). 19897575

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA

Information2

Donor DNAPhage P1 loxP, ECMV IRES, Aequorea victoria Venus, SV40 SV40 polyA, SV40 SV40 polyA promoter, E. coli neomycin
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood. 115(2), 230-237, (2010).
DepositorMasaru Taniguchi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data