Strain Data Sheet

RBRC10260

Information1

Image
BRC No.RBRC10260
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6J. Vα14 clone(iPS)
Former Common nameVa14 clone
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2014. iPS cells were established by introducing Yamanaka factors into NKT cells derived from B6J spleen. Chimeric mice were generated and then backcrossed with C57BL/6J for 10 generations.
Strain descriptionCloned mice harboring invariant TCR chain, Va14-Ja18.
Colony maintenance
ReferencesInt. Immunol., 26(10), 551-561 (2014). 24854340

Health Report

Examination Date / Room / Rack2022/08/08Room:2-検Rack:F
2022/05/09Room:2-検Rack:F
2022/02/21Room:2-検Rack:F
2021/11/22Room:2-検Rack:F
2021/08/23Room:2-検Rack:F
2021/05/24Room:2-検Rack:F
2021/03/08Room:2-検Rack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pou5f1POU domain, class 5, transcription factor 1UNPou5f1Oct3/4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Klf4Kruppel-like factor 4 (gut)UNKlf4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox2SRY (sex determining region Y)-box 2UNSox2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
NanogNanog homeoboxUNNanog

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Retrovirus LTRUN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNARetrovirus LTR, SV40 Poly A, Mouse PGK promoter, Mouse Oct3/4, Mouse Klf-4, Mouse Sox-2, Mouse Nanog.
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol. ;26(10), 551-561, (2014).
DepositorMasaru Taniguchi (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data