Strain Data Sheet

RBRC10250

Information1

Image
BRC No.RBRC10250
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Klre1<tm1Tgi>
Former Common nameNKG2I KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2004. R1 ES cells were used. This line was backcrossed with C57BL/6J for 6 generations.
Strain descriptionKlre1 (killer cell lectin-like receptor family E member 1) knockout mice. Homozygous mutants display impaired natural killer cell mediated allogeneic cytotoxicity. EGFP is placed downstream of translation initiation codon.
Colony maintenance
ReferencesBlood, 104, 781-783 (2004). 15069013

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Klre1killer cell lectin-like receptor family E member 16Klre1<tm1Tgi>targeted mutation 1, Masaru Taniguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAAequorea victoria EGFP cDNA, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Klre1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood 104, 781-783, (2004).
DepositorMasaru Taniguchi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data