Strain Data Sheet

RBRC10243

Information1

Image
BRC No.RBRC10243
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129-Scn2a<tm1Mml>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Prof. Maurice Montal, Section of Neurobiology, Division of Biological Sciences, University of California San Diego, USA. Deposited by Kazuhiro Yamakawa, RIKEN BSI in 2018. R1 ES cells[(129X1/SvJ x 129S1/Sv)F1] were used. C57BL/6J congenic line.
Strain descriptionScn2a (sodium channel, voltage-gated, type II, alpha)knockout mice. Homozygous mutants are lethal at postnatal day 1 or 2. Heterozygotes are fertile.
Colony maintenance
ReferencesBiophys. J., 78(6), 2878-2891 (2000). 10827969

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Scn2asodium channel, voltage-gated, type II, alpha2Scn2a<tm1Mml>targeted mutation 1, Mauricio MontalScn2a1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, mouse Scn2a genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biophys. J., 78(6), 2878-2891 (2000).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Prof. Maurice Montal, Section of Neurobiology, Division of Biological Sciences, University of California San Diego, USA, E-mail: mmontal@ucsd.edu) using the Approval Form.
2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEVELOPER (Prof. Maurice Montal) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuhiro Yamakawa (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data