Strain Data Sheet

RBRC10242

Information1

Image
BRC No.RBRC10242
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Scn1a<tm2.1Kzy>
Former Common name
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN BSI in 2007. E14 ES cells(129P2/OlaHsd) were used and the Neo gene was deleted by CAG-Flpe mice. C57BL/6J congenic line.
Strain descriptionFloxed mice for the Scn1a (sodium channel, voltage-gated, type I, alpha) gene. A pair of loxP sites flank Exon 7 of the Scn1a.
Colony maintenance
ReferencesHuman Molecular Genetics, 22(23), 4784-4804 (2013). 23922229

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Scn1asodium channel, voltage-gated, type I, alpha2Scn1a<tm2.1Kzy>targeted mutation 2.1, Kazuhiro Yamakawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Information2

Donor DNAphage P1 loxP sites, S.cerevisiae FRT(flipase recombination target) site, mouse Scn1a genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Human Molecular Genetics, 22(23), 4784-4804 (2013).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuhiro Yamakawa (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data