Strain Data Sheet

RBRC10241

Information1

Image
BRC No.RBRC10241
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6-Tg(Scn1a-GFP)1Kzy
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN BSI in 2008. In the BAC clone (RP23-232A20), GFP was placed at the translation initiation codon at the Exon 1 of the Scn1a gene. Then the BAC DNA construct was injected to the fertilized eggs of C57BL/6J. C57BL/6 genetic background.
Strain descriptionTransgenic mice expressing GFP underthe control of the Scn1a (sodium channel, voltage-gated, type I, alpha) gene promoter. Both males and females are fertile.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Scn1a promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAmouse Scn1a genomic DNA, jellyfish GFP cDNA, SV40 polyA, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-232A20
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuhiro Yamakawa (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data