Strain Data Sheet

RBRC10239

Information1

Image
BRC No.RBRC10239
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Parpbp<tm1.1Chum>
Former Common namePARI(Parpbp) knock-out
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shinichiro Chuma, Institute for Frontier Life and Medical Sciences, Kyoto University in 2017. KY1.1 ES cells derived from (129×C57BL/6)F1 were used. C57BL/6J congenic strain.
Strain descriptionParpbp (PARP1 binding protein) knockout mice. Homozygous mutants are fertile.
Colony maintenance
ReferencesMol. Cell Biol, 2017 Nov 13;37(23). pii: e00117-17. doi: 10.1128/MCB.00117-17. Print 2017 Dec 1. 28894029

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ParpbpPARP1 binding protein10Parpbp<tm1.1Chum>targeted mutation 1, Shinichiro Chuma

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Information2

Donor DNABacteriophage P1 LoxP site, mouse Parpbp genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Shinichiro Chuma) using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a teaching and academic research purpose.
DepositorShinichiro Chuma (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data