RBRC10238

Information1

Image
BRC No.RBRC10238
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tg(Cryd1/Cryaa-Sfrp2)1Jmca
Former Common nameSfrp2 lens (M2)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentTransgenic construct was injected into BCBF1 (C57/BL6xCBA) embryos. Generated by John McAvoy laboratory in the University of Sydney.After backcrossing to C57/BL6 line for a few generations, the line has been maintained as inbred.Small lens formation. Cataract development.Mice can be maintained as heterozygous or homozygous.
Strain descriptionTransgenic mouse line with Sfrp2 (secreted frizzled related protein-2) overexpression in lens.
Colony maintenance
ReferencesDevelopment Biology, 324, 161-176 (2008). 18824165

Health Report

Examination Date / Room / Rack2019/10/21Room:3-BRack:J
2019/08/26Room:3-BRack:J
2019/06/17Room:3-BRack:J
2019/04/15Room:3-BRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHhuman growth hormone polyAUNGH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sfrp2secreted frizzled-related protein 2UNSfrp2Chicken delta 1-crystallin (ASL1) enhancer and promoter, Mouse alpha A-crystallin (Cryaa) promoter, Rabbit beta-globin intron

Information2

Donor DNAChicken delta 1-crystallin (ASL1) enhancer and promoter, Mouse alpha A-crystallin (Cryaa) promoter, Rabbit beta-globin intron, Mouse Sfrp2 coding region, Human growth hormone poly A
Research applicationDevelopmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development Biology, 324, 161-176 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYuki Sugiyama (Okinawa Institute of Science and Technology Graduate University (OIST))
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data