Strain Data Sheet

RBRC10232

Information1

Image
BRC No.RBRC10232
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Reck<tm1Noda>
Former Common nameReck R2neo, Reck <Low>, Reck <Hypo>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited and developed by Makoto Noda, Kyoto University Graduate School of Medicine, in collaboration with RIKEN CDB. TT2 ES cells were used. This strain was backcrossed to C57BL/6J for 13 generations. C57BL/6 congenic strain.
Strain descriptionReck (reversion-inducing-cysteine-rich protein with kazal motifs) mutant mice. Exon 2 was floxed and FRT-pgk-neo-FRT was inserted at Intron 1. FRT-pgk-neo-FRT was not removed yet. Expression of RECK in homozyogous mice is less than 50% of that of wildtype mice.
Colony maintenance
ReferencesBiol. Open, 1(5),458-466 (2012). 23213437
BMC Dev. Biol., 10, 84 (2010). 20691046

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Reckreversion-inducing-cysteine-rich protein with kazal motifs4Reck<tm1Noda>targeted mutation 1, Makoto Noda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAYeast flippase recombination target sites (FRT), Mouse Phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, 3’-UTR, Bacteriophage P1 loxP sites, mouse Reck genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Open, 1(5),458-466 (2012).
DepositorMakoto Noda (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data