Strain Data Sheet

RBRC10221

Information1

Image
BRC No.RBRC10221
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameBALB/c-Tnfsf13b<em1Fjw>
Former Common nameBAFF-KO BALB/cA mice
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Wataru Fujii and Yasuyuki Goto, The University of Tokyo in 2017. Fertilized eggs derived from BALB/cAJcl was used. BALB/cAJcl genetic background.
Strain descriptionTnfsf13b [tumor necrosis factor (ligand) superfamily, member 13b] mutant mice generated by the CRISPR/Cas9. 738 bp genomic fragment covering exon 1 and 2 was deleted. Deleted genomic sequence, (Chr 8:10006808 to 10007545 (GRCm38.p4))CCAAAGGCCTAGACCTTCAAAGTGCTCCTCGTGGAATGGATGAGTCTGCAAAGACCCTGCCACCACCGTGCCTCTGTTTTTGCTCCGAGAAAGGAGAAGATATGAAAGTGGGATATGATCCCATCACTCCGCAGAAGGAGGAGGGTGCCTGGTTTGGGATCTGCAGGGATGGAAGGCTGCTGGCTGCTACCCTCCTGCTGGCCCTGTTGTCCAGCAGTTTCACAGCGATGTCCTTGTACCAGTTGGCTGCCTTGCAAGCAGACCTGATGAACCTGCGCATGGAGCTGCAGAGCTACCGAGGTTCAGCAACACCAGCCGCCGCGGGTGCTCCAGAGTTGACCGCTGGAGTCAAAGTGAGTTCACCCCTCCTCTGCCTGTGCAGGAGCCCCAGGTATGCCACTCCACAGCTGTCCAGTAACGGACTGGTTGTCTCTTTTCCTAGCTCCTGACACCGGCAGCTCCTCGACCCCACAACTCCAGCCGCGGCCACAGGAACAGACGCGCTTTCCAGGGACCAGAGGAAACAGGTACGGCTGGGGCACAGCAGTTAAGAGCTGGGGCTGTGCTGTGCACACCTGCAAGCCGCCCGGCCGCGGTGAACGAAGCACTTTGTGTAGCTTTGTAGCAATTGCAATAAGCTGCGTGGCGTGCCTTGCCCTACACTGAGCAGGGTGTATGGATAGGAGGATCCCTGTGGGAGGTGACAAGACGACTAGGGTGCACGGATCTCTCGCACTG
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun, 489(4), 528-533 (2017). 28583852

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:F
2022/11/29Room:4-BRack:F
2022/08/29Room:4-BRack:F
2022/05/31Room:4-BRack:F
2022/03/07Room:4-BRack:F
2021/12/06Room:4-BRack:F
2021/09/08Room:4-BRack:F
2021/06/07Room:4-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnfsf13btumor necrosis factor (ligand) superfamily, member 13b8Tnfsf13b<em1Fjw>endonuclease-mediated mutation 1, Wataru Fujii

Information2

Donor DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun, 489(4), 528-533 (2017).
DepositorYasuyuki Goto (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data