Strain Data Sheet

RBRC10208

Information1

Image
BRC No.RBRC10208
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Src<tm1(S75D)Goka>
Former Common nameSrcS75D, SrcSD
H-2 Haplotype
ES Cell lineCCE [129/Sv]
Background strain
Appearance
Strain developmentDeveloped by Goro Kato, University of Yamanashi in 2001. CCE ES cells derived from 129/SvEv were used. C57BL/6 congenic strain by backcrossing to C57BL/6N for 6 generations.
Strain descriptionMutant mice in which a point mutation in the Src gene was introduced at Serine 75 to convert it to Aspartic acid (S75D).
Colony maintenance
ReferencesJ. Biochem., 133, 563-569 (2003). 12801906
Sci. Rep., 7, 16779 (2017). 29196663

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SrcRous sarcoma oncogene2Src<tm1(S75D)Goka>

Information2

Donor DNAmouse Src genomic DNA (S75D mutant)
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorGoro Kato (University of Yamanashi)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data