Strain Data Sheet

RBRC10201

Strain Information

Image
BRC No.RBRC10201
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameCB17.Cg-Plxnb1<tm1Matl>
Former Common nameCB17-PlexinB1-KO
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Roland H. Friedel and deposited by Shinobu Inagaki, Osaka University Graduate School of Medicine, Division of Health Sciences. E14TG2a ES cells derived from 129P2/OlaHsd were used. This strain was backcrossed to C.B-17/lcr-+/+Jcl for 10 generations.
Strain descriptionPlxnb1 (plexin B1) knockout mice. C.B-17/lcr congenic strain.
Colony maintenance
References
Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes.
Friedel R H, Plump A, Lu X, Spilker K, Jolicoeur C, Wong K, Venkatesh T R, Yaron A, Hynes M, Chen B, Okada A, McConnell S K, Rayburn H, Tessier-Lavigne M
Proc. Natl. Acad. Sci. USA, 102(37) 13188-13193 (2005). 16129827

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA9
human Placental alkaline phosphatase cDNA9
CD4Rat CD4 cDNA9CD4
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)9En2
Iresinternal ribosomal entry site (EMCV)9
Plxnb1plexin B19Plxnb1targeted mutation 1, Marc Tessier-Lavigne
lacZbeta-galactosidase (E. coli)9
neoneomycin resistance gene9

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • nervous system phenotype(MP:0003631)

  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse En2 splice acceptor, Rat CD4 cDNA, E.coli Galactosidase/neomysin phosphotransferase fusion gene (β-geo), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human Placental alkaline phosphatase cDNA, SV40 Poly A, mouse Plxnb1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA 102: 13188-13193 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorDrs. Tatsuo Furuyama and Shinobu Inagaki (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data