Strain Data Sheet

RBRC10197

Information1

Image
BRC No.RBRC10197
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Trp53<em1.1Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentRIKEN BRC developed this strain by crossing a floxed Trp53 strain (RBRC09921) with a Cre strain. This was funded through the FY2016 AMED-NBRP fundamental technology upgrading program.
Strain descriptionTrp53 knockout mice in the C57BL/6N genetic background. A genomic region from Exon 2 to Exon 9 of the Trp53 was deleted.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/01/23Room:3-DRack:L
2022/10/25Room:3-DRack:L
2022/07/25Room:3-DRack:L
2022/04/25Room:3-DRack:L
2022/02/14Room:3-DRack:L
2021/11/15Room:3-DRack:L
2021/08/16Room:3-DRack:L
2021/05/17Room:3-DRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trp53transformation-related protein 5311Trp53<em1.1Rbrc>endonuclease mediated mutation 1.1, RIKEN BRC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Information2

Donor DNAPhage P1 loxP site, mouse Trp53 genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions1, In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to RIKEN BRC is requested.
2, For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
3, RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data