Strain Data Sheet

RBRC10196

Information1

Image
BRC No.RBRC10196
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(CAG-Tysnd1)Okazk>
Former Common nameTysnd1 Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Yasushi Okazaki, Saitama Medical University. C57BL/6 genetic background. ES cells derived from C57BL/6N were used. Mice were crossed with C57BL/6J.
Strain descriptionKockin mice overexpressing Tysnd1 (trypsin domain containing 1). CAG-Tysnd1 construct was knocked in at the ROSA26 locus.
Colony maintenance
ReferencesEMBO J., 26(3), 835-845 (2007). 17255948

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-Tysnd1)Okazk>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tysnd1trypsin domain containing 16Tysnd1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6

Information2

Donor DNAadenovirus splice acceptor site, Phage P1 loxP sites, E. coli neo, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mouse Tysnd1 cDNA, mouse Tysnd1 poly A tail, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 26(3), 835-845 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Use the following sentence, “We akcnowledge Y. Okazaki, C. Schönbach and Y. Mizuno for providing the Tysnd1 TG mice.”.
DepositorYasushi Okazaki (Saitama Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data