Strain Data Sheet

RBRC10195

Information1

Image
BRC No.RBRC10195
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBALB/c-Ffar4<tm1Kync>
Former Common nameGPR120KO mouse
H-2 Haplotype
ES Cell line
Background strainBALB/cCrSlc
Appearance
Strain developmentDeposited by Kazuo Inoue, Graduate School of Agriculture, Kyoto University. ES cells derived from BALB/c genetic background were used. BALB/c genetic background.
Strain descriptionFfar4, free fatty acid receptor (別名Gpr120, G-protein coupled receptor 120) knockout mice. Exon containing the translation initiation codon was replaced with the Neo gene. BALB/c genetic background. Homozygous mutants are viable and fertile and shown no gross abnormality.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:E
2022/11/29Room:4-BRack:E
2022/08/29Room:4-BRack:E
2022/05/31Room:4-BRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ffar4free fatty acid receptor 419Ffar4<tm1Kync>targeted mutation 1, Shigenobu Matsumura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEnterobacteria phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, mouse Pgk1 polyA, mouse Ffar4(GPR120) genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuo Inoue (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data