Strain Data Sheet

RBRC10186

Information1

Image
BRC No.RBRC10186
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Phf6<em1Utr>
Former Common namePhf6 floxed mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Satoru Miyagi, Graduate School of Medicine, Chiba University. Mice were generated by Laboratory Animal Resource Center, University of Tsukuba. Two loxP sites were introduced by the CRISPR technique using C57BL/6N-derived fertilized eggs. C56BL /6N genetic background.
Strain descriptionPhf6 (PHD finger protein 6)floxed mice. loxP sites were introduced at intron 3 and 5 of the Phf6 gene. Phf6 floxed mouse show no obvious phenotype.
Colony maintenance
ReferencesBlood, 33(23):2495-2506 (2019). 30917958

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Phf6PHD finger protein 6XPhf6<em1Utr>endonuclease-mediated mutation 1, University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Information2

Donor DNAP1 phage LoxP sites
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 33(23):2495-2506 (2019).
DepositorSatoru Miyagi (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Aug 2019
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data