Strain Data Sheet

RBRC10180

Information1

Image
BRC No.RBRC10180
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mboat4<tm1Shi>
Former Common nameGOAT-knockout mouse, GOAT-KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentBRUCE-4 ES cell line (derived from C57BL/6J mouse line) was used for homologous recombination. The mice were generated by Dr. Tomohiko Okuda et al. in Aburahi Laboratories of SHIONOGI & CO., LTD. in 2010. Chimeras generated using these ES cells were bred with C57BL/6JJcl (CLEA Japan, Inc.). After confirmation of germ-line transmission, homozygous knockout mice were generated by inbreeding of heterozygous mice. B6-Mboat4tm1Shi homozygotes were maintained via incrossing. The engineered mutation was transmitted to the progeny with the expected Mendelian ratio and develop normally.
Strain descriptionA null KO mouse of the Mboat4 gene encoding ghrelin O-acyltransferase (GOAT). Exon1 of the gene was replaced with a PGK-Neo cassette by homologous recombination in ES cells. Homozygous KO mice show no acylated ghrelin. This model mouse can be utilised for reserches of diseases, inter alia, metabolic syndrome.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesPeptides., ;86, 145-152 (2016). 27816752
J. Endocrinol., 228(2), 115-125 (2016). 26645250

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mboat4membrane bound O-acyltransferase domain containing 48Mboat4<tm1Shi>targeted mutation 1, Shionogi and Company, Ltd

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 phage loxP sites, Mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, SV40 polyA additional signal, mouse Mboat4 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J .Endocrinol., 228(2), 115-125 (2016).1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit academic institution for a not-for-profit academic research.
3. The Recipient must not provide any third party with the BIOLOGICAL RESOURCE without prior DEPOSITOR’s permission.
DepositorHirohide Nambu (Shionogi & Co., Ltd)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data