Strain Data Sheet

RBRC10171

Information1

Image
BRC No.RBRC10171
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>
Former Common nameRosa26-LSL-rtTA3/ Col-tetO-OSKM
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack2020/10/16Room:3-3Rack:D
2020/10/12Room:3-3Rack:D
2020/10/05Room:3-3Rack:D
2020/07/06Room:3-3Rack:D
2020/04/06Room:3-3Rack:D
2020/02/03Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Col1a1collagen, type I, alpha 111Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>targeted mutation 4, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3(rtTA3)Yasu>targeted mutation 3, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Information2

Donor DNAadenovirus type2 Splicing acceptor (SA) [Rosa26, Col1a1 locus], P1 Bacteriophage loxP sites, mouse Pgk1 promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin registance gene (Neo), SV40 pA, Escherichia coli K-12 strain derivative rtTA3, Escherichia coli K-12 strain derivative Hygromycin registance gene, CMV promoter, E. coli tetO (tetracycline operator), yeast Frt site, Escherichia coli ampicillin registance gene, mouse Oct3/4, mouse Sox2, mouse Klf4, mouse c-Myc, Porcineteschovirus-1 P2A,Thoseaasignavirus T2A,Equine rhinitis A virus E2A, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Commun., 9, 2081 (2018).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data