RBRC10171

Information1

Image
BRC No.RBRC10171
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>
Former Common nameRosa26-LSL-rtTA3/ Col-tetO-OSKM
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack2020/04/06Room:3-3Rack:D
2020/02/03Room:3-3Rack:D
2019/12/02Room:3-3Rack:D
2019/10/16Room:3-3Rack:D
2019/08/05Room:3-3Rack:D
2019/06/03Room:3-3Rack:D
2019/04/01Room:3-3Rack:D
2019/02/04Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Col1a1collagen, type I, alpha 111Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>targeted mutation 4, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3(rtTA3)Yasu>targeted mutation 3, Yasuhiro Yamada

Information2

Donor DNAadenovirus type2 Splicing acceptor (SA) [Rosa26, Col1a1 locus], P1 Bacteriophage loxP sites, mouse Pgk1 promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin registance gene (Neo), SV40 pA, Escherichia coli K-12 strain derivative rtTA3, Escherichia coli K-12 strain derivative Hygromycin registance gene, CMV promoter, E. coli tetO (tetracycline operator), yeast Frt site, Escherichia coli ampicillin registance gene, mouse Oct3/4, mouse Sox2, mouse Klf4, mouse c-Myc, Porcineteschovirus-1 P2A,Thoseaasignavirus T2A,Equine rhinitis A virus E2A, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Commun., 9, 2081 (2018).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Category I MTA: FP license of TBUSA

BRC mice in Publications

No Data