Strain Data Sheet

RBRC10168

Strain Information

Image
BRC No.RBRC10168
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dock2<tm1Tsas>
Former Common nameDOCK2KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshinori Fukui and Takehiko Sasazuki, Kyushu University in 2001. ES cells derived from 129/Sv were used. C57BL/6J congenic line. A neo gene replaced 3.1kb of genomic fragment that includes part of exon 3 through exon 5.
Strain descriptionDock2(dedicator of cyto-kinesis 2) knockout mice.
Colony maintenance
References
Haematopoietic cell-specific CDM family protein DOCK2 is essential for lymphocyte migration.
Fukui Y, Hashimoto O, Sanui T, Oono T, Koga H, Abe M, Inayoshi A, Noda M, Oike M, Shirai T, Sasazuki T
Nature, 412(6849), 826-831 (2001). 11518968

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA11
  • DOCK2 deficiency(MedGEN)
    • Dock2dedicator of cyto-kinesis 211Dock2targeted mutation 1, Takehiko Sasazuki
  • DOCK2 deficiency(MedGEN)
    • loxPphage P1 loxP11loxP
  • DOCK2 deficiency(MedGEN)
    • loxPphage P1 loxP11loxP
  • DOCK2 deficiency(MedGEN)
    • neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)
  • DOCK2 deficiency(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Peyer's patch morphology(MP:0000696)

  • abnormal cell migration(MP:0003091)

  • abnormal hematopoietic system morphology/development(MP:0002396)

  • abnormal immune system morphology(MP:0000685)

  • abnormal leukocyte migration(MP:0003156)
    • more 10 phenotypes
  • abnormal spleen morphology(MP:0000689)

  • abnormal spleen white pulp morphology(MP:0002357)

  • decreased T cell number(MP:0005018)

  • decreased lymphocyte cell number(MP:0005016)

  • decreased marginal zone B cell number(MP:0008182)

  • decreased mature B cell number(MP:0008211)

  • impaired neutrophil chemotaxis(MP:0008720)

  • increased susceptibility to xenobiotic induced morbidity/mortality(MP:0009766)

  • lymph node hypoplasia(MP:0008101)

  • spleen hypoplasia(MP:0000694)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse PGK promoter, E. coli neo, SV40 Poly A, mouse DOCK2 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    covid19_progression
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 412(6849), 826-831 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the　BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach　agreement on terms and conditions of use of it with DEPOSITOR and must obtain a　prior　written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in　the case of application for any patents or commercial use based on the results from　the use of the BIOLOGICAL RESOURCE.
    DepositorYoshinori Fukui (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data