Strain Data Sheet

RBRC10168

Information1

Image
BRC No.RBRC10168
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dock2<tm1Tsas>
Former Common nameDOCK2KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshinori Fukui and Takehiko Sasazuki, Kyushu University in 2001. ES cells derived from 129/Sv were used. C57BL/6J congenic line. A neo gene replaced 3.1kb of genomic fragment that includes part of exon 3 through exon 5.
Strain descriptionDock2(dedicator of cyto-kinesis 2) knockout mice.
Colony maintenance
ReferencesNature, 412(6849), 826-831 (2001). 11518968

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dock2dedicator of cytokinesis 211Dock2<tm1Tsas>targeted mutation 1, Takehiko Sasazuki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA11

Information2

Donor DNAPhage P1 loxP sites, mouse PGK promoter, E. coli neo, SV40 Poly A, mouse DOCK2 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
covid19_progression
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 412(6849), 826-831 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the　BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach　agreement on terms and conditions of use of it with DEPOSITOR and must obtain a　prior　written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in　the case of application for any patents or commercial use based on the results from　the use of the BIOLOGICAL RESOURCE.
DepositorYoshinori Fukui (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data