Strain Data Sheet

RBRC10167

Information1

Image
BRC No.RBRC10167
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gprc5c<tm1Yhir>
Former Common nameGPRC5C-deficient, (Acc. No. CDB0485K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentGenerated by RIKEN CDB(Acc. No. CDB0480K). TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Exon 2 was replaced with a fragment lacZ-neo. C57BL/6J congenic strain.
Strain descriptionGprc5c(G protein-coupled receptor, family C, group 5, member C)knockout mice. Mutants exhibit no obvious phenotype.
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 412(3), 460-465 (2011). 21840300

Health Report

Examination Date / Room / Rack2023/02/13Room:3-ARack:G
2023/01/10Room:3-ARack:G
2022/10/11Room:3-ARack:G
2022/07/11Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gprc5cG protein-coupled receptor, family C, group 5, member C11Gprc5c<tm1Yhir>targeted mutation 1, Yoshio Hirabayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA7

Information2

Donor DNAPhage P1 lox71 site, Phage P1 loxP site, E. coli lacZ gene, yeast FRT (flippase recombination target) sites, Mouse Phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Gprc5c genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYoshio Hirabayashi (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data