Strain Data Sheet

RBRC10166

Information1

Image
BRC No.RBRC10166
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gprc5b<tm1Yhir>
Former Common nameGPRC5B-deficient
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentGenerated by RIKEN CDB(Acc. No. CDB0480K). TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Exon 2 was replaced with a fragment lacZ-neo. C57BL/6J congenic strain.
Strain descriptionGprc5b(G protein-coupled receptor, family C, group 5, member B)knockout mice. Mutants exhibit suppression to obesity progression and insulin resistance induced by overeating. This strain is useful for research in obesity and type II diabetes. About 24 % mutants die by postnatal day 12. Deficit in motor learning.
Colony maintenance
ReferencesSci. Signal., 5(251), ra85 (2012). 23169819
Biochem. Biophys. Res. Commun., 412(3), 460-465 (2011). 21840300

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gprc5bG protein-coupled receptor, family C, group 5, member B7Gprc5b<tm1Yhir>targeted mutation 1, Yoshio Hirabayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA7

Information2

Donor DNAE. coli Neomycin resistance gene, E. coli Lac Z gene, Mouse Phosphoglycerate kinase promoter, SV40 Poly (A) signal, Phage P1 loxP, Yeast FRT
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYoshio Hirabayashi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data