Strain Data Sheet

RBRC10166

Strain Information

Image
BRC No.RBRC10166
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gprc5b<tm1Yhir>
Former Common nameGPRC5B-deficient
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentGenerated by RIKEN CDB(Acc. No. CDB0480K). TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Exon 2 was replaced with a fragment lacZ-neo. C57BL/6J congenic strain.
Strain descriptionGprc5b(G protein-coupled receptor, family C, group 5, member B)knockout mice. Mutants exhibit suppression to obesity progression and insulin resistance induced by overeating. This strain is useful for research in obesity and type II diabetes. About 24 % mutants die by postnatal day 12. Deficit in motor learning.
Colony maintenance
References
GPRC5B activates obesity-associated inflammatory signaling in adipocytes.
Kim Y J, Sano T, Nabetani T, Asano Y, Hirabayashi Y
Sci. Signal., 5(251), ra85 (2012). 23169819

Comparative characterization of GPRC5B and GPRC5CLacZ knockin mice; behavioral abnormalities in GPRC5B-deficient mice.
Sano T, Kim Y J, Oshima E, Shimizu C, Kiyonari H, Abe T, Higashi H, Yamada K, Hirabayashi Y
Biochem. Biophys. Res. Commun., 412(3), 460-465 (2011). 21840300

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA7
Frtyeast FRT (flippase recombination target) site7Frt
Frtyeast FRT (flippase recombination target) site7Frt
Frtyeast FRT (flippase recombination target) site7Frt
Gprc5bG protein-coupled receptor, family C, group 5, member B7Gprc5btargeted mutation 1, Yoshio Hirabayashi
lacZbeta-galactosidase (E. coli)7
loxPphage P1 loxP7loxP
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal acute inflammation(MP:0002498)

  • abnormal behavior(MP:0004924)

  • abnormal brown adipose tissue thermogenesis(MP:0011699)

  • abnormal motor learning(MP:0002804)

  • decreased body weight(MP:0001262)
    • more 14 phenotypes
  • decreased circulating glucose level(MP:0005560)

  • decreased circulating leptin level(MP:0005668)

  • decreased fat cell size(MP:0009269)

  • decreased inflammatory response(MP:0001876)

  • decreased liver weight(MP:0003402)

  • decreased locomotor activity(MP:0001402)

  • decreased total body fat amount(MP:0010025)

  • impaired synaptic plasticity(MP:0001900)

  • improved glucose tolerance(MP:0005292)

  • increased circulating insulin level(MP:0002079)

  • increased insulin secretion(MP:0003058)

  • increased oxygen consumption(MP:0005289)

  • neonatal lethality, incomplete penetrance(MP:0011088)

  • postnatal lethality, incomplete penetrance(MP:0011086)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, E. coli Lac Z gene, Mouse Phosphoglycerate kinase promoter, SV40 Poly (A) signal, Phage P1 loxP, Yeast FRT
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorYoshio Hirabayashi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data