RBRC10162

Information1

Image
BRC No.RBRC10162
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameR26-WntVis(Accession No.CDB0303K)
Former Common nameR26-WntVis(Accession No.CDB0303K)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesGenes Cells., 21(6):661-9 (2016). 27030109

Health Report

Examination Date / Room / Rack2020/06/01Room:3-BRack:
2020/02/17Room:3-BRack:I
2019/12/16Room:3-BRack:I
2019/10/21Room:3-BRack:I
2019/08/26Room:3-BRack:I
2019/06/17Room:3-BRack:I
2019/04/15Room:3-BRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(TCF/Lef-H2B/EGFP)Knd>targeted mutation 1.1, Hisato Kondo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
H2Bhuman histone H2B cDNA6H2Bherpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA6HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal6

Information2

Donor DNAmouse TCF/Lef binding site, herpes simplex virus thymidine kinase promoter (HSV tk promoter), human H2B cDNA, Jellyfish EGFP cDNA, SV40 polyA signal, rabbit b-globin pA, yeast FRT (flippase recombination target) site, mouse Gt(ROSA)26Sor region genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 21(6):661-9 (2016).(1) When publishing the research results obtained by the use of the BIOLOGICAL RESOURCE, the RECIPIENT shall refer to the Accession No. CDB0303K and URL (www2.clst.riken.jp/arg/mutant%20mice%20list.html) in the manuscript. (2) The RECIPIENT shall inform the DEPOSITOR (mutant.bdr@riken.jp, kondohh@cc.kyoto-su.ac.jp, and takemoto.tatsuya@tokushima-u.ac.jp) of any publications with the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data