RBRC10151

Information1

Image
BRC No.RBRC10151
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus molossinus
Strain nameMSM-A<em1Ogu>
Former Common name
H-2 Haplotype
ES Cell line
Background strainMSM/Ms
Appearance
Strain development
Strain description
Colony maintenance
ReferencesSci. Rep., 7:42476, 2017. doi:10.1038/srep42476 28195201

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
anonagouti2a<em1Ogu>

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 7:42476, 2017. doi:10.1038/srep42476
DepositorAtsuo Ogura (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources
Lab HP

BRC mice in Publications

No Data