Strain Data Sheet

RBRC10146

Information1

Image
BRC No.RBRC10146
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Actb-roGFP1)91Awolf
Former Common nameCyto-roGFP mice(CRO91)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Alexnder M Wolf, G Nippon Medical School in 2012. Transgenic construct was injected into fertilized eggs derived from C57BL/6J. Mice were backcrossed to C57BL/6J for more than 10 generations. Mice were maintained as heterozygote.
Strain descriptionTransgenic mice expressing ratiometric redox-sensitive GFP (roGFP) in the cytosol of several tissue under the control of chicken beta-actin promoter. Redox states can be monitored at liver and other tissues.
Colony maintenance
ReferencesJ. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roGFP1redox-sensing Green Fluorescent Protein (Aequorea victoria)UNroGFP1chicken beta-actin promoter

Information2

Donor DNAChicken beta-Actin promoter, Aquorea Victoria GFP variant (C48S/Q80R/S147C/Q204C) cDNA, Rabbit beta-globin PolyA signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAlexander M. Wolf (Nippon Medical School)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data