Strain Data Sheet
RBRC10145
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10145 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Tppp3<tm1(EGFP)Yasu> |
| Former Common name | Tppp3-EGFP |
| H-2 Haplotype | |
| ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from ((C57BL/6 x 129S4/SvJae)F1) were used. Mixed genetic background. |
| Strain description | Knock-in mouse of EGFP to the 3’UTR of the Tppp3 locus. |
| Colony maintenance | |
| References | Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas. Komura S, Ito K, Ohta S, Ukai T, Kabata M, Itakura F, Semi K, Matsuda Y, Hashimoto K, Shibata H, Sone M, Jo N, Sekiguchi K, Ohno T, Akiyama H, Shimizu K, Woltjen K, Ozawa M, Toguchida J, Yamamoto T, Yamada Y Nat. Commun., 10(1):3999 (2019). 31488818Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision） |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| SV40 polyA signal | 8 | ||||||
| Bsd | Aspergillus terreus blasticidin resistance gene (Bsd) | 8 | Bsd | mouse phosphoglycerate kinase promoter (PGK promoter) | |||
| EGFP | Green Fluorescent Protein (Aequorea victoria) | 8 | EGFP | ||||
| HBB2 | rabbit beta-globin polyA | 8 | HBB2 | ||||
| Ires | internal ribosomal entry site (EMCV) | 8 | |||||
| Tppp3 | tubulin polymerization-promoting protein family member 3 | 8 | Tppp3 | targeted mutation 1, Yasuhiro Yamada | |||
| rox | Bacteriophage D6 rox site | 8 | rox | ||||
| rox | Bacteriophage D6 rox site | 8 | rox | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Aequorea victoria EGFP cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Tppp3 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Yasuhiro Yamada (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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