RBRC10145

Information1

Image
BRC No.RBRC10145
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tppp3<tm1(EGFP)Yasu>
Former Common nameTppp3-EGFP
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNat. Commun., 10(1):3999 (2019). 31488818
Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. (in revision)

Health Report

Examination Date / Room / Rack2020/02/17Room:3-DRack:C
2019/12/16Room:3-DRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tppp3tubulin polymerization-promoting protein family member 38Tppp3<tm1(EGFP)Yasu>targeted mutation 1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
BsdAspergillus terreus blasticidin resistance gene (Bsd)8Bsdmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA8HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roxD6 Bacteriophage rox site8rox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roxD6 Bacteriophage rox site8rox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal8

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Aequorea victoria EGFP cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Tppp3 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data