Strain Data Sheet

RBRC10144

Strain Information

Image
BRC No.RBRC10144
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tppp3<tm2(cre/ERT2)Yasu>
Former Common name
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from ((C57BL/6 x 129S4/SvJae)F1) were used. Mixed genetic background.
Strain descriptionKnock-in mouse of CreERT2 to the 3’UTR of the Tppp3 locus. Cre/loxP recombination can be induced by the administration of Tamoxifen.
Colony maintenance
References
Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas.
Komura S, Ito K, Ohta S, Ukai T, Kabata M, Itakura F, Semi K, Matsuda Y, Hashimoto K, Shibata H, Sone M, Jo N, Sekiguchi K, Ohno T, Akiyama H, Shimizu K, Woltjen K, Ozawa M, Toguchida J, Yamamoto T, Yamada Y
Nat. Commun., 10(1):3999 (2019). 31488818

Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision）

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal8
BsdAspergillus terreus blasticidin resistance gene (Bsd)8Bsdmouse phosphoglycerate kinase promoter (PGK promoter)
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)8ERT2
HBB2rabbit beta-globin polyA8HBB2
Iresinternal ribosomal entry site (EMCV)8
Tppp3tubulin polymerization-promoting protein family member 38Tppp3targeted mutation 2, Yasuhiro Yamada
crePhage P1 Cre recombinase8cre
roxBacteriophage D6 rox site8rox
roxBacteriophage D6 rox site8rox

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Tppp3 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1):3999 (2019). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Cre-ERT(2): NOTICE TO RECIPIENT
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data