RBRC10143

Information1

Image
BRC No.RBRC10143
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Sox10<tm1(cre/ERT2)Yasu>
Former Common nameSox10-CreERT2
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNat. Commun., 10(1):3999 (2019). 31488818
Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. (in revision)

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sox10SRY (sex determining region Y)-box 1015Sox10<tm1(cre/ERT2)Yasu>targeted mutation 1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
BsdAspergillus terreus blasticidin resistance gene (Bsd)15Bsdmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)15ERT2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA15HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase15cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roxD6 Bacteriophage rox site15rox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roxD6 Bacteriophage rox site15rox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal15

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Sox10 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

No Data