RBRC10142

Information1

Image
BRC No.RBRC10142
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae> Col1a1<tm2(tetO-EWSR1/ATF1,-mCherry)Yasu>
Former Common nameRosa26-M2rtTA/Col-tetO-EWS/ATF1
H-2 Haplotype
ES Cell lineKH2[F1:C57BL/6 x 129Sv]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNat. Commun., 10(1):3999 (2019). 31488818
Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. (in revision) 31488818
J. Clin. Invest., 123, 600-610, doi:10.1172/jci63572 (2013). 23281395

Health Report

Examination Date / Room / Rack2020/06/01Room:3-ARack:F
2020/02/17Room:3-ARack:F
2019/12/16Room:3-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)11mCherry

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae>targeted mutation 1, Rudolf Jaenisch

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
hyghygromycin phasphotransferase (E. coli)6hyg

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)6puro

Information2

Donor DNAEscherichia coli K-12 M2rtTA, Rabbit globin polyA, mouse PGK promoter, Streptomyces alboniger Puromycin resistance gene, mouse Rosa genomic DNA,adenovirus type 2 Splicing acceptor (SA), yeast Frt site, E. coli tetO (tetracycline operator), CMV promoter, human EWS cDNA, human ATF1 cDNA, FLAG tag(synthetic), Influenza virus A HA tag, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA, Escherichia coli K-12 hygromycin resistance gene, Escherichia coli ampicillin resistant gene, mouse Col1A1 genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Category I MTA: FP license of TBUSA

BRC mice in Publications

No Data