Strain Data Sheet

RBRC10136

Information1

Image
BRC No.RBRC10136
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Col1a1<tm2(tetO-Dek)Hirt>
Former Common nameiDek
H-2 Haplotype
ES Cell lineKH2[F1:C57BL/6 x 129Sv]
Background strain
Appearance
Strain developmentDeveloped by Hiroyuki Tomita, Gifu University in 2010. KH2 ES cell (F1:C57BL/6 x 129/SV) was used. Mice were backcrossed to C57BL/6J for 3 or 4 generations.
Strain descriptionA mouse strain in which cDNA coding Dek [DEK oncogene (DNA binding)] with FLAG tag is driven by the TetO promoter. DNA construct was knocked in at the Col1a1 (collagen, type I, alpha 1) locus.
Colony maintenance
ReferencesCancer Med. 2017 Aug 23. doi: 10.1002/cam4.1157. 28834425

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Col1a1collagen, type I, alpha 111Col1a1<tm2(tetO-Dek)Hirt>targeted mutation 2, Hiroyuki Tomita

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DekDEK oncogene (DNA binding)11DekE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)11FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hyghygromycin phasphotransferase (E. coli)11Hygmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SAAdenovirus Major late transcript splice acceptor sequence11SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA11

Information2

Donor DNAyeast FRT (flipase recombination target) sites, Adenovirus Major late transcript splice acceptor sequence (SA), Simian Virus 40 SV40 polyA, E. coli tetO (tetracycline operator), Mouse Dek cDNA, Synthetic FLAG tag, vector, Escherichia coli ampicillin resistant gene, mouse PGK promotor, E. coli hygromycin resistance gene, mouse Col1a1 genomic DNA
Research applicationFLP/frt system
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Med. 2017 Aug 23. doi: 10.1002/cam4.1157.
DepositorHiroyuki Tomita (Gifu University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data