RBRC10115

Information1

Image
BRC No.RBRC10115
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Gdpd4<em1Osb>
Former Common nameGdpd4<-19/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27. 31455729

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gdpd4glycerophosphodiester phosphodiesterase domain containing 47Gdpd4<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Gdpd4 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )
Lab HP

BRC mice in Publications

No Data