Strain Data Sheet
RBRC10109
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10109 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Slc6a5<tm1.1(cre)Ksak> |
| Former Common name | GLYT2-Cre knockin mice |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Deposited by Toshiakizu Kakizaki, Gunma University Graduate School of Medicine in 2017. RENKA ES cells derived from C57BL/6N was used. A neo gene was removed by crossing with a Flp mouse line. C57BL/6 genetic background. |
| Strain description | Cre knock-in mouse at the translation initiation codon of the Slc6a5 (Glyt2) gene. Homozygous mice are lethal by 2 weeks after their birth. |
| Colony maintenance | |
| References | A glycine transporter 2-Cre knock-in mouse line for glycinergic neuron-specific gene manipulation. Kakizaki T, Sakagami H, Sakimura K, Yanagawa Y IBRO Reports, 3, 9-16 (2017). 30135938 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/09/08Room:3-CRack:KSentinel mouse program 2025/06/30Room:3-CRack:KSentinel mouse program 2025/04/21Room:3-CRack:KSentinel mouse program 2025/01/20Room:3-CRack:KSentinel mouse program 2024/11/13Room:3-CRack:KAdditional tests 2024/10/21Room:3-CRack:KSentinel mouse program 2024/07/22Room:3-CRack:KSentinel mouse program 2024/04/22Room:3-CRack:KSentinel mouse program 2024/01/22Room:3-CRack:KSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| Slc6a5 MGI:105090 | solute carrier family 6 (neurotransmitter transporter, glycine), member 5 | 7 | Slc6a5<tm1.1(cre)Ksak> | targeted mutation 1.1, Kenji Sakimura | |||
| cre | Phage P1 Cre recombinase | 7 | cre | ||||
| human granulocyte colony-stimulating factor gene polyA | 7 | ||||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | phage P1 Cre, human granulocyte colony-stimulating factor gene polyA, yeast FRT (flipase recombination target) site, mouse Slc6a5 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Cre driver |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. IBRO Reports, 3, 9-16 (2017). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations. |
| Depositor | Toshikazu Kakizaki (Gunma University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Dec 2019 |
BRC mice in Publications |
|---|
Brown ST, Holla MR, Land MA, Yang S, McDonald AJ, St-Pierre F, Raman IM. High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement. Cell Rep 44(8) 116148(2025) 40811060 |
Wu XM, Lu B, He JY, Zhang YX, Wu ZY, Xiong ZQ. Aberrant outputs of glutamatergic neurons in deep cerebellar nuclei mediate dystonic movements. Sci Adv 11(19) eadp2377(2025) 40344058 |
Wei XP, Collie M, Dempsey B, Fortin G, Yackle K. A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing. Neuron 110(4) 644-657.e6(2022) 34998469 |