Strain Data Sheet

RBRC10109

Information1

Image
BRC No.RBRC10109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Slc6a5<tm1.1(cre)Ksak>
Former Common nameGLYT2-Cre knockin mice
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Toshiakizu Kakizaki, Gunma University Graduate School of Medicine in 2017. RENKA ES cells derived from C57BL/6N was used. A neo gene was removed by crossing with a Flp mouse line. C57BL/6 genetic background.
Strain descriptionCre knock-in mouse at the translation initiation codon of the Slc6a5 (Glyt2) gene. Homozygous mice are lethal by 2 weeks after their birth.
Colony maintenance
ReferencesIBRO Reports, 3, 9-16 (2017). 30135938

Health Report

Examination Date / Room / Rack2022/10/25Room:3-CRack:K
2022/07/25Room:3-CRack:K
2022/04/25Room:3-CRack:K
2022/02/14Room:3-CRack:K
2021/11/15Room:3-CRack:K
2021/08/16Room:3-CRack:K
2021/07/18Room:3-CRack:K
2021/05/17Room:3-CRack:K
2021/05/14Room:3-CRack:K
2021/02/15Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc6a5solute carrier family 6 (neurotransmitter transporter, glycine), member 57Slc6a5<tm1.1(cre)Ksak>targeted mutation 1.1, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase7cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
human granulocyte colony-stimulating factor gene polyA7

Information2

Donor DNAphage P1 Cre, human granulocyte colony-stimulating factor gene polyA, yeast FRT (flipase recombination target) site, mouse Slc6a5 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. IBRO Reports, 3, 9-16 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations.
DepositorToshikazu Kakizaki (Gunma University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Dec 2019
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Wei XP, Collie M, Dempsey B, Fortin G, Yackle K.
A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing.
Neuron (2021) 34998469