RBRC10096
Information1 | |
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| Image | |
| BRC No. | RBRC10096 |
| Type | CRISPR/Cas9 |
| Species | Mus musculus |
| Strain name | C57BL/6J-Hr<em1Utr> |
| Former Common name | Hrem1Utr/em1Utr |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Exp. Anim., 66(4):437-445 (2017). 28717054 |
Health Report | |
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| Examination Date / Room / Rack | 2020/12/07Room:2-検Rack:C 2020/09/07Room:2-検Rack:C 2020/06/08Room:2-検Rack:C 2020/03/09Room:2-検Rack:C 2020/01/06Room:2-検Rack:C 2019/11/11Room:2-検Rack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hrhairless14Hr<em1Utr>endonuclease-mediated mutation 1, University of Tsukuba Laboratory Animal Resource Center |
Information2 | |
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| Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim. 2017 Jul 18. doi: 10.1538/expanim.17-0049. |
| Depositor | Fumihiro Sugiyama (University of Tsukuba) |
| Strain Status |  Live mice Frozen sperm |
| Strain Availability | Live mouse (3 to 6 months) |
| Additional Info. | Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English ) Mouse of the Month Feb 2020 |
BRC mice in Publications |
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| No Data |