Strain Data Sheet

RBRC10095

Information1

Image
BRC No.RBRC10095
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Irf3<tm2.1Ttg>
Former Common nameIRF3-flox
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tadatsugu Taniguchi and Hideyuki Yanai, University of Tokyo in 2017. TT2 ES cells derived from C57BL/6 x CBA F1 were used. The neo gene was deleted by crossing mice expressing the Flippase. Mice were backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain.
Strain descriptionFloxed IRF-3(Irf3:interferon regulatory factor)mice. Homozygous mice are viable.
Colony maintenance
ReferencesProc. Natl. Acad .Sci. USA, 115(20), 5253-5258 (2018). 29712834

Health Report

Examination Date / Room / Rack2022/06/13Room:3-3Rack:A
2022/03/14Room:3-3Rack:A
2021/12/14Room:3-3Rack:A
2021/09/13Room:3-3Rack:A
2021/06/14Room:3-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Irf3interferon regulatory factor 37Irf3<tm2.1Ttg>targeted mutation 2.1, Tadatsugu Taniguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Irf3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad .Sci. USA, 115(20), 5253-5258 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB1028K and Web Site (www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. The RECIPIENT must submit any and all copy of the publications using BIOLOGICAL RESOURCES to RIKEN Center for Life Sciences Technologies, Laboratories of Animal Resource development and Genetic Engineering (RIKEN CLST/LARGE) (mutant.bdr@riken.jp).
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the first major literature is requested.
DepositorTadatsugu Taniguchi (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Nov 2020
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data