Strain Data Sheet

RBRC10084

Information1

Image
BRC No.RBRC10084
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Zfyve27<tm1Kei>
Former Common namePrt KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Keiichi Nakayama, Medical Institute of Bioregulation, Kyusyu University in 2017. E14(129P2/OlaHsd) ES cell line was used. C57BL/6 congenic strain by backcrossing for 7 generations.
Strain descriptionZfyve27 (zinc finger, FYVE domain containing 27, Protrudin) knockout mouse. Mutation in Zfyve27 (Protrudin) has been found in patients of the hereditary spastic paraplegia. This mutant mouse exhibits neurological symptoms resembling mental illness. Exon 1 and 2 were replaced with the Neo gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zfyve27zinc finger, FYVE domain containing 2719Zfyve27<tm1Kei>targeted mutation 1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA19

Information2

Donor DNABacteriophage P1 LoxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Zfyve27 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data