Strain Data Sheet

RBRC10084

Strain Information

Image
BRC No.RBRC10084
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Zfyve27<tm1Kei>
Former Common namePrt KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Keiichi Nakayama, Medical Institute of Bioregulation, Kyusyu University in 2017. E14(129P2/OlaHsd) ES cell line was used. C57BL/6 congenic strain by backcrossing for 7 generations.
Strain descriptionZfyve27 (zinc finger, FYVE domain containing 27, Protrudin) knockout mouse. Mutation in Zfyve27 (Protrudin) has been found in patients of the hereditary spastic paraplegia. This mutant mouse exhibits neurological symptoms resembling mental illness. Exon 1 and 2 were replaced with the Neo gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zfyve27
MGI:1919602
zinc finger, FYVE domain containing 2719Zfyve27<tm1Kei> targeted mutation 1, Keiichi I Nakayama
  • hereditary spastic paraplegia 33(MedGEN)
  • loxP phage P1 loxP19loxP
    loxP phage P1 loxP19loxP
    neo neomycin resistance gene (E. coli)19 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA19

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal behavior(MP:0004924)

  • abnormal dendritic spine morphology(MP:0009936)

  • abnormal neurite morphology(MP:0008415)

  • athetotic walking movements(MP:0001527)

  • behavior/neurological phenotype(MP:0005386)
  • more 8 phenotypes
  • behavioral despair(MP:0002573)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased startle reflex(MP:0001489)

  • hindlimb paralysis(MP:0000755)

  • hyperactivity(MP:0001399)

  • impaired cued conditioning behavior(MP:0009456)

  • nervous system phenotype(MP:0003631)
  • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 LoxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Zfyve27 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Contact info: Dr. Michiko Shirane, Ph.D., Nagoya City University <shiram@phar.nagoya-cu.ac.jp>
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Arora A, Kivelä AM, Wang L, Minkeviciene R, Taskinen JH, Zhang B, Koponen A, Sun J, Shirane M, Zhou Y, Hotulainen P, Raiborg C, Olkkonen VM.
    Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.
    Cell Mol Life Sci 79(4) 220(2022) 35368213