Strain Data Sheet

RBRC10083

Information1

Image
BRC No.RBRC10083
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Map3k5<tm3.1Hijo>
Former Common nameAsk1 flox/flox, Map3k5 flox/flox
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. ENKA (C57BL/6) ES cells were used. C57BL/6 genetic background.
Strain descriptionFloxed Map3k5 (mitogen-activated protein kinase kinase kinase 5、ASK1) mice. Exon 15 was flanked by loxP sites.
Colony maintenance
ReferencesNat. Commun., 7:11158 (2016). doi: 10.1038/ncomms11158. 27045525
Kamiyama et al, 2017 accepted 28753204

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k5mitogen-activated protein kinase kinase kinase 510Map3k5<tm3.1Hijo>targeted mutation 3.1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Information2

Donor DNAPhage P1 loxP sites, Saccharomyces cerevisiae FRT site, mouse Map3k5 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data