RBRC10081

Information1

Image
BRC No.RBRC10081
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm1(CAG-NGFR*)Mtac>
Former Common nameR26-CAG-LoxP-hCD271
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesGenesis, 53:387-393 (2015). 26012873

Health Report

Examination Date / Room / Rack2020/06/01Room:3-CRack:L
2020/02/17Room:3-CRack:L
2019/12/16Room:3-CRack:L
2019/10/21Room:3-CRack:L
2019/08/26Room:3-CRack:L
2019/06/17Room:3-CRack:L
2019/04/15Room:3-CRack:L
2019/02/18Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-NGFR*)Mtac>targeted mutation 1, Makoto Tachibana

Gene symbolGene nameChr.Allele symbolAllele namePromoter
CD2716CD271CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
STOP6STOP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)6puro

Information2

Donor DNAStreptomyces alboniger puromycin resistance gene, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, human CD271 gene cDNA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 53(6), 387-393 (2015).
DepositorMakoto Tachibana (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data