Strain Data Sheet

RBRC10075

Information1

Image
BRC No.RBRC10075
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ggcx<tm1Sino>
Former Common nameGGCX flox/flox, Floxed GGCX allele
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Satoshi Inoue, Saitama Medical University (2014). ES cells derived from 129P2/OlaHsd were used. Mice were backcrossed to C57BL/6N over 8 generations.
Strain descriptionThese mice possess loxP sites flanking a neo gene and exon 6 of the GGCX gene. This strain is useful for generating conditional mutations of the GGCX gene.
Colony maintenance
ReferencesPLoS One. 2014 Feb 10;9(2):e88643. doi: 10.1371/journal.pone.0088643. eCollection 2014. 24520408

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ggcxgamma-glutamyl carboxylase6Ggcx<tm1Sino>targeted mutation 1, Satoshi Inoue

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAバクテリオファージ loxp配列(バクテリオファージP1由来ゲノムDNA), 大腸菌 Neo耐性遺伝子(大腸菌由来cDNA), マウスphosphoglycerate kinase 1 遺伝子プロモーター(マウス由来ゲノムDNA)
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorSatoshi Inoue (Saitama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data